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KMID : 0545120070170010096
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 1 p.96 ~ p.103
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Two Bacterial Entophytes Eliciting Both Plant Growth Promotion and Plant Defense on Pepper (Capsicum annuum L.)
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Kang Seung-Hoon
Cho Hyun-Soo
Cheong Hoon
Ryu Choong-Min
Kim Ji-Hyun
Park Seung-Hwan
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Abstract
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Plant growth-promoting rhizobacteria (PGPR) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. Some of the PGPR have been reported to be able to enter plant tissues and establish endophytic populations. Here, we demonstrate an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against bacterial pathogens. Initially, out of 150 bacterial isolates collected from healthy stems of peppers cultivated in the Chungcheong and Gyeongsang provinces of Korea, 23 putative endophytic isolates that were considered to be predominating and representative of each pepper sample were selected. By phenotypic characterization and partial 16S rDNA sequence analysis, the isolates were identified as species of Ochrobacterium, Pantoea, Pseudomonas, Sphingomonas, Janthinobacterium, Ralstonia, Arthrobacter, Clavibacter, Sporosarcina, Acidovorax, and Brevundimonas. Among them, two isolates, PS4 and PS27, were selected because they showed higher and more consistent colonizing capacity in pepper stems at the levels of 106 -107 CFU/g tissue, and were found to be most closely related to Pseudomonas rhodesiae and Pantoea ananatis, respectively, by additional analyses of their entire 16S rDNA sequences. Drenching application of the two strains on the pepper seedlings promoted significant growth of peppers, enhancing their root fresh weight by as much as 73.9% and 41.5%, respectively. The two strains also elicited significant resistance of plants against Xanthomonas axonopodis pv. vesicatoria.
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KEYWORD
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Pseudomonas rhodesiae, Pantoea ananatis, bacterial endophyte, plant growth-promoting rhizobacteria, induced systemic resistance, pepper
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